DEVELOPMENT AND VALIDATION OF NEW COLORIMETRIC METHOD FOR THE ESTIMATION OF ALOGLIPTIN & LOTEPREDNOL

Raval Kashyap
Research Scholar, Department of Pharmacy, Pacific University, Udaipur, Rajasthan, India.
U. Srinivasa
Head of Department, Srinivas College of pharmacy, Mangalore, Karnataka, India.

Alogliptin ,Loteprednol ,NaOH ,HCL ,UV spectrophotometric ,Validation Parameters ,

Colorimetric methods have been developed for the estimation of Alogliptin & Loteprednol. Here Methods were based on reaction involving the formation of orange &blue colored complex with NQS(1, 2 napthoquinone -4-sulfonic acid sodium) in the presence of alkaline medium for Alogliptin & Ammonium molibdate in presence of acidic medium for Loteprednol. The maximum absorbance of the solution was measured at 468nm& 745 nm against blank respectively. The calibration curve calculated obeys Beer’s law over the concentration range of 200-600 & 10-50µg/ml.The method was validated based on ICH guidelines. The high recovery and low relative standard deviation confirms the suitability of the method for determination Alogliptin & Loteprednol. Hence it is useful for the routine analysis of Alogliptin&Loteprednol.

5 , 2 , 2015